The Dna Extraction Essay Example for Free

The Dna Extraction Essay 1. The salt contributes positively charged atoms that neutralise the normal negative charge of DNA. Salt is used at a high molarity due to the fact that it precipitates all of the proteins out. DNA is insoluble in low molar salt solutions but soluble in low molar salt solutions thus keeping the DNA in solution. 2. blending the onion will homogenize the mixture and it helps with the breakdown of the cell walls. Blending saves one the time and effort of using a motar and pestle, however it may break a lot of the DNA which is not favourable as one needs a lot of DNA for the extraction. 3. The enzymes in the soap are used to break down the lipid (fat) molecules of the cells nuclear membranes releasing the contents of the cell crucially including the DNA. These enzymes in the soap are what break down grease while washing dishes. 4. The DNA does not dissolve in this alcohol but rather pushes up through and out of the solution or precipitates. It is less dense than water or cell scum which is what settles to the bottom of the glass so it floats up into the alcohol layer, where you see it as a snotty, string-like substance, with small bubbles formed on it. 5. Because protein is stored in them for the nutrition of the new plants. 6. It is too small to be seen with the naked eye. What you extracted is millions of strands of DNA. In addition to that, whilst the substance was heated, the DNA got denatured which results in it looking more like a ladder than a helix. 7. Most of the DNA extracted during this proccess comes from the nucleus of the cell. AIM: The purpose of this experiment is to extract DNA from a variety of cells (Onion cells in particular) and see DNA molecules. This will show that, contrary to popular opinion, DNA is not just found in blood cells, but in a variety of tissues. Prior knowledge should include the fact that cell membranes are layers of lipids, or fat molecules, that DNA is found in the nucleus of a cell, and that enzymes speed up chemical reactions. HYPOTHESIS: DNA is present in the cells of all living organisms. METHOD: 1. Prepare two water baths one at 60Â °C and another filled with ice and water, around 4Â °C. For the hot water bath, a large metal pot can be used along with a thermometer with an appropriate temperature range. For the ice bath, a mixing bowl filled with ice and water works well. 2. For each onion, make a solution consisting of 10 ml of liquid dishwashing detergent and 1.5 g of table salt. Put in a 250 ml beaker and emulsify. 3. Add distilled water to make a final volume of 100 ml. Dissolve the salt by stirring slowly to avoid foaming. 4. Coarsely chop one large onion with a food processor or blender and put into a 1000 ml mixing bowl. For best results, do not chop the onion too finely. The size of the pieces should be like those used in making spaghetti. It is better to have the pieces too large than too small. 5. Cover chopped onion with the 100 ml of solution from step 2. The liquid detergent causes the cell membrane to break down and dissolves the lipids and proteins of the cell by disrupting the bonds that hold the cell membrane together. The detergent causes lipids and proteins to precipitate out of the solution. Salt enables nucleic acids to precipitate out of an alcohol solution because it shields the negative phosphate end of DNA, causing the DNA strands to come closer together and coalesce. 6. Put the measuring cup in a hot water bath at 60Â °C for 10-12 minutes. During this time, press the chopped onion mixture against the side of the measuring cup with the back of the spoon. (Do not keep the mixture in the hot water bath for more than 15 minutes because the DNA will begin to break down.) If using a large metal pot for water bath, remove the pot from the stove before placing the onion-containing measuring cup inside—the procedure is safer if the pot is off the burner. Cont inue to monitor temperature of water bath and make adjustments as needed. 7. The heat treatment softens the phospholipids in the cell membrane and denatures the DNAse enzymes which, if present, would cut the DNA into small fragments so that it could not be extracted. 8. Cool the mixture in an ice water bath for 5 minutes. During this time, press the chopped onion mixture against the side of the measuring cup with the back of the spoon. This step slows the breakdown of DNA. 9. Filter the mixture through a #6 coffee filter or four layers of cheese cloth placed in a strainer over a 4-cup measuring cup. When you filter the onion mixture, try to keep the foam from getting into the filtrate. It sometimes filters slowly, so you might want to put the whole set up in the refrigerator and let it filter overnight. 10. Dispense the onion solution into a test tube. The test tube should contain about 1 teaspoon of solution or be about 1/3 full. For most uniform results among test tubes, stir the solution frequently when dispensing it into the tubes. There is not an adva ntage to dispensing more than one teaspoon of solution into a test tube. The solution can be stored in a refrigerator for about a day before it is used for the laboratory exercise. When the solution is removed from the refrigerator, it should be gently mixed before the test tubes are filled. 11. Add cold alcohol to the test tube to create an alcohol layer on top of about 1 cm. For best results, the alcohol should be as cold as possible. The alcohol can be added to the solution in at least three ways: (a) Fill a pasteur pipette with alcohol, put it to bottom of the test tube, and release the alcohol. (b) Or, put about 1 cm of alcohol into the bottom of a test tube and add the onion solution. (c) Or, slowly pour the alcohol down the inside of the test tube with a pasteur pipette or medicine dropper. DNA is not soluble in alcohol. When alcohol is added to the mixture, all the components of the mixture, except for DNA, stay in solution while the DNA precipitates out into the alcohol layer. 1 2. Let the solution sit for 2-3 minutes without disturbing it. It is important NOT to shake the test tube. You can watch the white DNA precipitate out into the alcohol layer. When good results are obtained, there will be enough DNA to spool on to a glass rod, a pasteur pipette that has been heated at the tip to form a hook, or similar device. A wooden skewer or nut pick (small metal rod with curved tip) may also work well for spooling DNA if Pasteur pipette is unavailable. DNA has the appearance of white mucus. CONCLUSION: With these findings we can conclude that DNA is present and can be found in the cells of all living organisms and not just in those of the human body.

The Champion Lands :: Free Descriptive Essay About A Place

The Champion Lands The former Champion Lands of Vermont consist of 132,000 acres in the Northeast Kingdom of Vermont. The Northeast Kingdom of Vermont has some of Vermont's most extensive areas of relitively remote and wild lands. A substantial portion of the Champion Lands are located in the the Nulhegan Basin, an extensive area of northern lowland forest and wetlands ringed by hills and mountians of moderatr elevation and drained by the Nulhegan River. The Champion Lands of Vermont are part of a larger system known as the Northern Forest. Stretching 400 miles from Lake Ontario to the Atlantic Ocean, the Northern Forest covers more than 25 million acres across New York's Tug Hill plateau and Adirondack Mountains and includes nearly all of northern Vermont, New Hampshire, and Maine. The forest reaches north and east into Quebec and the Maritime Provinces of Canada. This extensive regional forest contains a range of forest age-classes, from early successional to, in a few isolated locations, mature forest, but it is by and large young forest, less than 100 years old. It provides important habitat for the large mammals native to the extensive northeastern deciduous, coniferous and mixed forests. These include black bear, bobcat, deer and moose. The lands are divided into three different ownership parcels, each area has its own unique features and area of interest The West Mountain WMA lands are dominated by three major features: in the center of the parcel, West Mountain rises to an elevation of 2,733 feet above sea level; to the north and east the land drains into a series of small ponds in the Wheeler Stream drainage, while to the west and south Paul Stream drains an area dominated by Ferdinand Bog. These two stream drainages, which are tributaries of the Connecticut River, contain what is thought to be the greatest concentration of glacial ice-contact deposits in Vermont. The result is a highly varied terrain containing kames, kame moraines, eskers, and kettles surrounding the resistant granite of West Mountain. Notch Pond Mountain, part of the Nulhagan Basin mountainous rim to the north of West Mountain, separates the Wheeler Stream and Paul Stream drainages from the Nulhegan River. The mountains and high hills on the West Mountain WMA are strongly dominated by northern hardwood forests, while the stream drainages are dominated by red spr uce-hardwood forest or lowland spruce-fir forests, and a variety of wetlands and ponds. The wetlands are predominantly northern white cedar swamps, spruce-fir-tamarack swamps, and alder-beaver meadow complexes.

Is the Internet a Blessing or a Curse for the Millenial Generation?

Name: Olamide Basorun E-mail address: [email  protected] com Telephone number: 080258693012 Is the internet a Blessing or Curse for the Millennial Generation? By: Olamide Basorun Internet has been, perhaps, the most outstanding innovation in the field of communication in the history of mankind. As with every single innovation, the internet has its own advantages and disadvantages. However, I believe that there is a greater magnitude of advantages and these undoubtedly outweigh the disadvantages. During his evolutionary journey from Neanderthal to Homo erectus and then to Homo sapiens, man has come a long way.Because of his continuous quest for more and more amenities and facilities, he has been able to invent many new things. The nineties have seen a major turn around with the invention of computers. Until, a few years back, the term Internet was practically synonymous to most of the people. But today, the Internet has become the most powerful communication tool for people througho ut the world. With the advent of Internet, our earth has virtually reduced and has attained the form of a global village. The Internet has now become the best business tool in the modern scenario.Today, it has brought the world in one single room. Right from getting news across the corner of the world to accessing knowledge resources to shopping, everything is at your fingertips. The Internet has tremendous potential and a lot to offer in terms of services such as faster communication. The foremost target of Internet has always been speedy communication and it has excelled way beyond the expectations. Newer innovations are only going to make it faster and more reliable. Now, you can communicate in a fraction of second with a person who is sitting in the other part of the world.For more personal and interactive communication, you can avail the facilities of chat services, video conferencing and so on. Besides, there are plenty of messenger services in offering. With the help of such services, it has become very easy to establish a kind of global friendship where you can share your thoughts and explore other cultures. Moreover, the internet is an excellent avenue to collect information resources. Information is probably the biggest advantage that the Internet offers. The internet is a virtual treasure trove of information.Any kind of information on any topic under the sun is available on the Internet. The search engines like Google, Yahoo are at your service on the Internet. There is a huge amount of information available on the Internet for just about every subject known to man, ranging from government law and services, trade fairs and conferences, market information, new ideas and technical support, the list is simply endless. Students and children are among the top users who surf the Internet for research. Today, it is almost required that students should use it for research or the purpose of gathering resources.Even teachers have started giving assignments t hat require extensive research on the Internet. Besides, you can have an access to the latest researches in the field of medicine, technology and so on. Numerous web sites such as America's Doctor also allow you to talk to the doctors online and receive medical diagnosis without going through the stress of going to a hospital and avoid the embarrassment of face to face consultations. Entertainment is another popular raison d'etre why many people prefer to surf the Internet.In fact, the Internet has become quite successful in trapping the multifaceted entertainment industry. Downloading games or just surfing the celebrity websites are some of the uses people have discovered. Even celebrities are using the Internet effectively for promotional campaigns. Besides, there are numerous games that can be downloaded for free. The industry of online gaming has tasted dramatic and phenomenal attention by game lovers. The Internet has made life very convenient. With numerous online services you can now perform all your transactions online.You can book tickets for a movie, transfer funds, pay utility bills, taxes etc. , right from your home. Some travel websites even plan an Itinerary as per your preferences and take care of airline tickets, hotel reservations etc. Also the concept of e-commerce is used for any type of commercial manoeuvring or business deals that involves the transfer of information across the globe via the Internet. It has become a phenomenon associated with any kind of shopping, business deal etc. You name a service, and e-commerce with its giant tentacles engulfing every single product and ervice will make it available at your doorstep. Websites such as eBay allow you to even bid for homes, buy, sell or auction stuff online. As much as the internet is exceptionally useful it has its own disadvantages such as theft of personal information if you use the Internet for online banking, social networking or other services, you may risk a theft to your person al information such as name, address, credit card number and so on. Unscrupulous people can access this information through unsecured connections or by planting software and then use your personal details for their benefit.Needless to say, this may land you in serious trouble. Also the issue of spamming presents a serious problem for the internet. Spamming refers to sending unwanted e-mails in bulk, which provide no purpose and needlessly obstruct the entire system. Such illegal activities can be very frustrating for you as it makes your Internet slower and less reliable. Along with these problems Internet users are often plagued by virus attacks on their systems. Virus programs are inconspicuous and may get activated if you click a seemingly harmless link.Computers connected to the Internet are prone to targeted virus attacks and may end up crashing. Pornography is causing a lot of problems for under aged internet users . Pornography is perhaps the biggest disadvantage of the Inter net. Internet allows you to access and download millions of pornographic photos, videos and other X-rated files. Such unrestricted access to porn can be detrimental for children and teenagers. It can even place havoc in the marital and social lives of adults. Thanks to the Internet, people now not only meet on social networks.More and more people are getting engulfed in virtual world and drifting apart from their friends and family. Even children prefer to play online games rather than going out and mingling with other kids. This may hamper a healthy social development in children. Thus, the Internet has the potential to make your life simple and convenient, as well as wreak havoc in your life. Its influence is mostly dictated by the choices you make while you are online. With clever use, you can manage to harness its unlimited potential. Is the Internet a Blessing or a Curse for the Millenial Generation? Name: Olamide Basorun E-mail address: [email  protected] com Telephone number: 080258693012 Is the internet a Blessing or Curse for the Millennial Generation? By: Olamide Basorun Internet has been, perhaps, the most outstanding innovation in the field of communication in the history of mankind. As with every single innovation, the internet has its own advantages and disadvantages. However, I believe that there is a greater magnitude of advantages and these undoubtedly outweigh the disadvantages. During his evolutionary journey from Neanderthal to Homo erectus and then to Homo sapiens, man has come a long way.Because of his continuous quest for more and more amenities and facilities, he has been able to invent many new things. The nineties have seen a major turn around with the invention of computers. Until, a few years back, the term Internet was practically synonymous to most of the people. But today, the Internet has become the most powerful communication tool for people througho ut the world. With the advent of Internet, our earth has virtually reduced and has attained the form of a global village. The Internet has now become the best business tool in the modern scenario.Today, it has brought the world in one single room. Right from getting news across the corner of the world to accessing knowledge resources to shopping, everything is at your fingertips. The Internet has tremendous potential and a lot to offer in terms of services such as faster communication. The foremost target of Internet has always been speedy communication and it has excelled way beyond the expectations. Newer innovations are only going to make it faster and more reliable. Now, you can communicate in a fraction of second with a person who is sitting in the other part of the world.For more personal and interactive communication, you can avail the facilities of chat services, video conferencing and so on. Besides, there are plenty of messenger services in offering. With the help of such services, it has become very easy to establish a kind of global friendship where you can share your thoughts and explore other cultures. Moreover, the internet is an excellent avenue to collect information resources. Information is probably the biggest advantage that the Internet offers. The internet is a virtual treasure trove of information.Any kind of information on any topic under the sun is available on the Internet. The search engines like Google, Yahoo are at your service on the Internet. There is a huge amount of information available on the Internet for just about every subject known to man, ranging from government law and services, trade fairs and conferences, market information, new ideas and technical support, the list is simply endless. Students and children are among the top users who surf the Internet for research. Today, it is almost required that students should use it for research or the purpose of gathering resources.Even teachers have started giving assignments t hat require extensive research on the Internet. Besides, you can have an access to the latest researches in the field of medicine, technology and so on. Numerous web sites such as America's Doctor also allow you to talk to the doctors online and receive medical diagnosis without going through the stress of going to a hospital and avoid the embarrassment of face to face consultations. Entertainment is another popular raison d'etre why many people prefer to surf the Internet.In fact, the Internet has become quite successful in trapping the multifaceted entertainment industry. Downloading games or just surfing the celebrity websites are some of the uses people have discovered. Even celebrities are using the Internet effectively for promotional campaigns. Besides, there are numerous games that can be downloaded for free. The industry of online gaming has tasted dramatic and phenomenal attention by game lovers. The Internet has made life very convenient. With numerous online services you can now perform all your transactions online.You can book tickets for a movie, transfer funds, pay utility bills, taxes etc. , right from your home. Some travel websites even plan an Itinerary as per your preferences and take care of airline tickets, hotel reservations etc. Also the concept of e-commerce is used for any type of commercial manoeuvring or business deals that involves the transfer of information across the globe via the Internet. It has become a phenomenon associated with any kind of shopping, business deal etc. You name a service, and e-commerce with its giant tentacles engulfing every single product and ervice will make it available at your doorstep. Websites such as eBay allow you to even bid for homes, buy, sell or auction stuff online. As much as the internet is exceptionally useful it has its own disadvantages such as theft of personal information if you use the Internet for online banking, social networking or other services, you may risk a theft to your person al information such as name, address, credit card number and so on. Unscrupulous people can access this information through unsecured connections or by planting software and then use your personal details for their benefit.Needless to say, this may land you in serious trouble. Also the issue of spamming presents a serious problem for the internet. Spamming refers to sending unwanted e-mails in bulk, which provide no purpose and needlessly obstruct the entire system. Such illegal activities can be very frustrating for you as it makes your Internet slower and less reliable. Along with these problems Internet users are often plagued by virus attacks on their systems. Virus programs are inconspicuous and may get activated if you click a seemingly harmless link.Computers connected to the Internet are prone to targeted virus attacks and may end up crashing. Pornography is causing a lot of problems for under aged internet users . Pornography is perhaps the biggest disadvantage of the Inter net. Internet allows you to access and download millions of pornographic photos, videos and other X-rated files. Such unrestricted access to porn can be detrimental for children and teenagers. It can even place havoc in the marital and social lives of adults. Thanks to the Internet, people now not only meet on social networks.More and more people are getting engulfed in virtual world and drifting apart from their friends and family. Even children prefer to play online games rather than going out and mingling with other kids. This may hamper a healthy social development in children. Thus, the Internet has the potential to make your life simple and convenient, as well as wreak havoc in your life. Its influence is mostly dictated by the choices you make while you are online. With clever use, you can manage to harness its unlimited potential.

The Story of an Hour and the Necklace Essay - 2858 Words

RUNNING HEAD: THE STORY OF AN HOUR amp; THE NECKLACE 1 The Story of an Hour amp; The Necklace Cindy Rohwer ENG 125 Douglas Goss September 8, 2012 THE STORY OF AN HOUR amp; THE NECKLACE 2 How little a thing is needed for us to be saved? Both short stories, Maupassant’s â€Å"The Necklace† and Chopin’s â€Å"The Story of an†¦show more content†¦Louise’s life has been devoid of any kind of emotion, but she weeps in wild abandonment, when she hears of her husband’s death. Although she is not stuck as many women would have been by a paralyzed inability to except the painful sense of loss (Jamil, 2009). The pretense of emotion signifies Louise’s sensitivity, responsiveness and the mindfulness of her situation. For one climatic hour of Louise’s life, she truly tastes joy. For one hour of emotion, she does glimpse the meaning and fulfillment of her life. â€Å"To be fully alive, means to engage in heightened consciousness, to observe and connect with the world around one’s self† (Jamil, 2009). Chopin makes clear that to simply observe the world through one’s rational faculty is nowhere near as powerful as observing it with the vibrant, vigorous, acute and heightened awareness that emotion makes possibl In the play form of â€Å"The Necklace† for children, THE STORY OF AN HOUR amp; THE NECKLACE 4 Mathilde is shown around and was being shown off by Madame Ambassador, as if Mathilde was her daughter. Mathilde spent the evening in a cloud of happiness and it was four in the morning before they left the ball (de Maupassant, 2002). Guy de Maupassant’s â€Å"The Necklace† has stood the test of time and isShow MoreRelatedEssay on Comparing â€Å"the Story of an Hour† and â€Å"the Necklace†1632 Words   |  7 PagesComparing â€Å"The Story of an Hour† and â€Å"The Necklace† Short stories are always very enjoyable to read. They are very concise but mostly have such a deep message resonating throughout the text that the reader is left pondering over an extension of the story to imagine the possible ending if they were novels instead. The works under consideration are meant to be compared because of their unique theme which reflects how two authors can write similar yet distinctive stories in different times. Kate Chopin’sRead MoreThe Necklace and Story of an Hour; a Comparison Essay2800 Words   |  12 PagesThe Necklace and Story of An Hour; A Comparison Megan Ford ENG125 Instructor Reljic August 19, 2012 At first glance, Chopin’s Story of an Hour (1894) and de Maupassant’s The Necklace (1884), appear to have very little in common. Chopin’s story, as displayed in its title is quite short; while in comparison, de Maupassant tells a much more detailed account of the beleaguered Loisel’s, who must learn from the self-centred Madam Loisel. With de Maupassant’sRead MoreRelationship Between the Necklace and the Story of an Hour Essays779 Words   |  4 PagesENGISH 1121 The Story of an Hour and The Necklace share many similarities and also many differences; both explore the feeling that both wives harbor towards their husbands and the lack of communication that both wives share. In this essay I will discuss the similarities and differences that the two short stories share with regards to communication. In Guy de Maupassants The Necklace is the story of Mathilde Loisel, who resents her station in life. Mathilde Loisel is shown to be a vainRead More The Womans Rose, The Necklace, and The Story of an Hour Essay799 Words   |  4 PagesThe Womans Rose, The Necklace, and The Story of an Hour In this essay I will be comparing ‘The Woman’s Rose’ By Olive Schreiner, ‘The Story Of An Hour’ By Kate Chopin and ‘The Necklace’ By Guy De Maupassant. Each of these stories arte set in the patriarchal 19th century and all of them have the hint of women fighting for freedom, for example in ‘The Story Of An Hour’, Mrs Mallard didn’t feel free until the death of her husband, Mr Mallard; ‘said it over and over under her breath: â€Å"freeRead MoreNineteenth Century Gender Roles Exposed in The Necklace and The Story of an Hour2020 Words   |  9 Pagesrepresentation of The Necklace and The story of an hour represents gender roles as defined by the nineteenth century society guidelines . The The Story of an Hour was set in the late nineteenth century in the home of Louise Mallard.(Kate Chopin).It was written on April 19, 1894,by Kate Chopin. The theme Many also focus on women revolt against conformity, often against gender conformity or against social norms that limit omens possibilities in life. 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However she could not have certain things that cost too much because she did not have the money to buy the items she longed for. One night her husband brought home an invitation to a ball but she was not happy about this because she did not have a dress good enough to attend